8ZEB
Crystal structure of BCL-XL bound by cp-B6X-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-24 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.022, 97.605, 93.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.950 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yaa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.606 | 2.036 |
| High resolution limit [Å] | 1.837 | 1.837 |
| Number of reflections | 21539 | 3017 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289.15 | 15% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 10,000 |
