8ZCK

Serial Femtosecond Crystallography Structure of Fc Fragment of Human IgG1 from Biosimilar VEGF-Trap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	298
Detector technology	CCD
Collection date	2023-10-27
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 21 21 21
Unit cell lengths	50.470, 79.990, 143.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 2.000
R-factor	0.1896
Rwork	0.189
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	CrystFEL
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.670	2.140
High resolution limit [Å]	2.000	2.000
Number of reflections	132583	7163
<I/σ(I)>	4.49
Completeness [%]	100.0	100
Redundancy	135.3
CC(1/2)	0.960	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.1M Bis-Tris pH 6.5, 25% w/v Polyethylene glycol 3350