8ZC8

The structure of MitM and mitomycin A with SAH in mitomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	110
Detector technology	CCD
Collection date	2023-11-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97861
Spacegroup name	P 31 2 1
Unit cell lengths	113.953, 113.953, 108.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.340 - 2.800
R-factor	0.2258
Rwork	0.224
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zc7
RMSD bond length	0.013
RMSD bond angle	1.472
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	20544	2957
<I/σ(I)>	14.6
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% w/v PEG 3350, 10% w/v Ethylene glycol, 0.2 M Sodium sulfate