8ZAX
Crystal structure of a short-chain dehydrogenase from Lactobacillus fermentum with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.827, 88.818, 157.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.540 |
| R-factor | 0.21883 |
| Rwork | 0.217 |
| R-free | 0.24772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.150 | 1.204 |
| Rmeas | 0.154 | |
| Rpim | 0.034 | 0.322 |
| Total number of observations | 1348050 | |
| Number of reflections | 71115 | 6829 |
| <I/σ(I)> | 2.6 | 1.3 |
| Completeness [%] | 92.6 | 90.1 |
| Redundancy | 19 | 13.3 |
| CC(1/2) | 0.998 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M MES pH 6.0, 0.05M calcium chloride, 43% PEG 200 |
