8Z9L
Crystal structure of SARS-CoV-2 RBD bound to Rhinolophus affinis ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.736, 129.479, 563.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.013 - 3.598 |
| R-factor | 0.2436 |
| Rwork | 0.242 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.727 |
| High resolution limit [Å] | 3.598 | 3.598 |
| Number of reflections | 55023 | 5181 |
| <I/σ(I)> | 4.67 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11 | |
| CC(1/2) | 0.965 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M HEPES pH7.5, 10% w/v Polyethylene 8000, 8% v/v Ethylene glycol |
