8Z8W

Crystal Structure of EphA6/Caskin1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97891
Spacegroup name	P 43 2 2
Unit cell lengths	70.434, 70.434, 74.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.800 - 3.300
R-factor	0.1991
Rwork	0.196
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.778
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.118	0.036	0.916
Rmeas	0.125	0.038	0.965
Rpim	0.039	0.012	0.296
Total number of observations	30909
Number of reflections	3116	196	154
<I/σ(I)>	4.1
Completeness [%]	99.8	100	100
Redundancy	9.9	8.7	10.5
CC(1/2)	0.992	0.999	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	2.2 M Sodium chloride, 0.1 M Tris pH 8.5