8Z8V

Crystal structure of human serum albumin in complex with ALB8(VHH) domain of ozoralizumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	60.290, 60.900, 62.810
Unit cell angles	89.51, 63.72, 76.49

Refinement procedure

Resolution	45.300 - 2.050
R-factor	0.20432
Rwork	0.202
R-free	0.23854
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.518
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	2.170
High resolution limit [Å]	2.050	2.050
Number of reflections	47566	7440
<I/σ(I)>	7.09
Completeness [%]	97.6
Redundancy	3.9
CC(1/2)	0.994	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.7	293	100 mM SPG Buffer (pH4.7) 21 % PEG1500