8Z3B

Crystal structure of GSTd2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	58.124, 82.355, 106.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.355 - 2.300
Rwork	0.185
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.934
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_360)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.355	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	23353	2288
<I/σ(I)>	8.8
Completeness [%]	99.8
Redundancy	7.2
CC(1/2)	0.840	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	15% PEG3350, and 0.12M Tris at a pH of 7.0