8YZT
Crystal structure of the BANP BEN domain in complex with its target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.995, 58.060, 214.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 2.580 |
| R-factor | 0.2289 |
| Rwork | 0.225 |
| R-free | 0.30460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.984 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.360 | 2.640 |
| High resolution limit [Å] | 2.580 | 2.600 |
| Rmerge | 0.139 | 0.591 |
| Number of reflections | 23248 | 1109 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | containing 0.02 M citric acid, 0.08 M BIS-Tris propane (pH: 8.8) and 16% w/v polyethylene glycol 3,350 |
