8YYJ
RNase J2 mutant H80A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-10-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.890, 131.910, 116.190 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 66.000 - 3.000 |
| Rwork | 0.247 |
| R-free | 0.29980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.180 | 0.540 |
| Number of reflections | 35724 | 5223 |
| <I/σ(I)> | 5.2 | 2 |
| Completeness [%] | 91.3 | 92.7 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.960 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 293 | 100mM tri-sodium citrate dihydrate pH 5.6, 20% PEG 4000, 20% Isopropanol, 40% Formamide |
