8YXN
X-ray structure of Clostridium perfringens autolysin catalytic domain in the P1 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-19 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.130, 47.520, 64.310 |
| Unit cell angles | 94.79, 89.92, 96.29 |
Refinement procedure
| Resolution | 36.503 - 1.600 |
| Rwork | 0.163 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.530 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 56867 | 4154 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.995 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% (w/v) PEG 3000, 100mM CHES/ sodium hydroxide |
