8YXB
Crystal structure of the HSA complex with ceftriaxone and myristate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.893, 93.786, 94.747 |
| Unit cell angles | 105.73, 100.03, 89.71 |
Refinement procedure
| Resolution | 38.260 - 2.200 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.386 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.714 |
| Number of reflections | 63358 | 10070 |
| <I/σ(I)> | 17.76 | 2.32 |
| Completeness [%] | 98.1 | 96.5 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 32% PEG 3350, 50mM potassium phosphate pH 7.0 |
