8YXA
Crystal structure of the HSA complex with cefazolin and myristate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.801, 94.351, 94.461 |
| Unit cell angles | 105.19, 101.78, 90.03 |
Refinement procedure
| Resolution | 37.900 - 2.500 |
| R-factor | 0.2701 |
| Rwork | 0.268 |
| R-free | 0.31890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.476 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 | 0.694 |
| Number of reflections | 42775 | 6786 |
| <I/σ(I)> | 11.1 | 1.22 |
| Completeness [%] | 99.9 | 96.4 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 32% PEG 3350, 50mM potassium phosphate pH 7.0 |
