8YWB

a protein with a ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-08-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	89.337, 81.264, 73.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.920 - 2.490
Rwork	0.284
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.920	2.579
High resolution limit [Å]	2.490	2.490
Number of reflections	18527	1841
<I/σ(I)>	13.5
Completeness [%]	99.2
Redundancy	2
CC(1/2)	0.999	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	Sodium phosphate monobasic, HEPES/Sodium hydroxide, Potassium phosphate dibasic