8YV4

The crystal structure of G.acetivorans RNA kinase Ark1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-24
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	53.870, 63.297, 70.220
Unit cell angles	90.00, 109.46, 90.00

Refinement procedure

Resolution	26.860 - 1.870
R-factor	0.1929
Rwork	0.191
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.790	1.970
High resolution limit [Å]	1.870	1.870
Number of reflections	35482	35482
<I/σ(I)>	11.7
Completeness [%]	96.2
Redundancy	5.7
CC(1/2)	0.992	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289.15	0.1M tri-Sodium citrate pH5.5 20%(w/v) PEG 3000