8YUE

Crystal structure of the kinesin-14 motor protein from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	164.506, 67.617, 94.196
Unit cell angles	90.00, 96.99, 90.00

Refinement procedure

Resolution	46.750 - 3.150
R-factor	0.2532
Rwork	0.251
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.574
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.500	3.320
High resolution limit [Å]	3.100	3.100
Rpim	0.138	1.000
Number of reflections	17466	2747
<I/σ(I)>	6.9
Completeness [%]	96.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.5M NaCl, 50mM NaPi, pH6.8, 7mM DTT, 13% PEG 8000