8YTQ
The structure of apoCopC from Thioalkalivibrio paradoxus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.084, 144.787, 55.350 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 55.350 - 1.700 |
| R-factor | 0.18644 |
| Rwork | 0.184 |
| R-free | 0.23063 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.155 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.350 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.612 |
| Rmeas | 0.099 | 0.700 |
| Rpim | 0.048 | 0.336 |
| Total number of observations | 194184 | 10364 |
| Number of reflections | 47498 | 2495 |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 98.2 | |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.995 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Ammonium sulfate |
