8YRU
Crystal structure of D-amino acid transaminase from Haliscomenobacter hydrossis (apo form) after 15 sec of soaking with phenylhydrazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-16 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.490, 71.780, 52.241 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 21.430 - 2.000 |
| R-factor | 0.17928 |
| Rwork | 0.177 |
| R-free | 0.23214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.799 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.430 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.546 |
| Rmeas | 0.092 | 0.615 |
| Rpim | 0.038 | 0.276 |
| Total number of observations | 119567 | 6358 |
| Number of reflections | 21346 | 1390 |
| <I/σ(I)> | 16.8 | 2.8 |
| Completeness [%] | 99.0 | |
| Redundancy | 5.6 | 4.6 |
| CC(1/2) | 0.998 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 45% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 |
