8YRT
Crystal structure of D-amino acid transaminase from Haliscomenobacter hydrossis in the holo form obtained at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-30 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.043, 71.902, 52.015 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 21.960 - 2.000 |
| R-factor | 0.14716 |
| Rwork | 0.145 |
| R-free | 0.19038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.285 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.960 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.391 |
| Rmeas | 0.081 | 0.425 |
| Rpim | 0.032 | 0.164 |
| Total number of observations | 139425 | 9847 |
| Number of reflections | 21090 | 1484 |
| <I/σ(I)> | 23.7 | 4.9 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.998 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 45% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 |
