8YRS
Crystal structure of human RECQ1 helicase containing a flexible linker in complex with tailed duplex DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-12-22 |
Detector | MAR CCD 300 mm |
Wavelength(s) | 0.978930 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.323, 96.573, 96.806 |
Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
Resolution | 46.640 - 2.430 |
R-factor | 0.2386 |
Rwork | 0.237 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wwy |
RMSD bond length | 0.012 |
RMSD bond angle | 1.414 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.280 | 2.570 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.106 | 1.040 |
Rmeas | 0.124 | 1.220 |
Rpim | 0.064 | 0.628 |
Number of reflections | 35793 | 359 |
<I/σ(I)> | 9.6 | 1.3 |
Completeness [%] | 92.9 | 53.6 |
Redundancy | 3.8 | |
CC(1/2) | 0.997 | 0.536 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2 M Sodium malonate (pH=7.0) and 22% PEG 3350 |