8YRS

Crystal structure of human RECQ1 helicase containing a flexible linker in complex with tailed duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2023-12-22
Detector	MAR CCD 300 mm
Wavelength(s)	0.978930
Spacegroup name	P 1 21 1
Unit cell lengths	83.323, 96.573, 96.806
Unit cell angles	90.00, 106.88, 90.00

Refinement procedure

Resolution	46.640 - 2.430
R-factor	0.2386
Rwork	0.237
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wwy
RMSD bond length	0.012
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.280	2.570
High resolution limit [Å]	2.430	2.430
Rmerge	0.106	1.040
Rmeas	0.124	1.220
Rpim	0.064	0.628
Number of reflections	35793	359
<I/σ(I)>	9.6	1.3
Completeness [%]	92.9	53.6
Redundancy	3.8
CC(1/2)	0.997	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2 M Sodium malonate (pH=7.0) and 22% PEG 3350