8YRL

Crystal structure of Aspergillus fumigatus Galactofuranosylransferase (AfGfsA) in complex with UDP and galactofuranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	84.142, 188.606, 71.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.340 - 2.370
R-factor	0.209
Rwork	0.206
R-free	0.26820
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.492
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	2.460
High resolution limit [Å]	2.370	2.370
Number of reflections	47069	4542
<I/σ(I)>	14.4	2.55
Completeness [%]	99.5
Redundancy	7.3
CC(1/2)	0.999	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris (pH 5.5), 28% (w/v) polyethylene glycol 3350