8YR5
Crystal structure of E. coli phosphatidylserine synthase in apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.040, 94.100, 194.750 |
| Unit cell angles | 88.24, 87.70, 60.07 |
Refinement procedure
| Resolution | 48.640 - 2.830 |
| R-factor | 0.24394 |
| Rwork | 0.242 |
| R-free | 0.28478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 2.880 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.187 | 1.860 |
| Rmeas | 0.207 | 2.063 |
| Rpim | 0.088 | 0.882 |
| Total number of observations | 722456 | 35555 |
| Number of reflections | 135369 | 6672 |
| <I/σ(I)> | 8.1 | 1 |
| Completeness [%] | 98.2 | |
| Redundancy | 5.3 | 5.3 |
| CC(1/2) | 0.995 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 297 | 0.09 M Sodium citrate tribasic dihydrate pH 5.9, 0.09 M Lithium sulfate monohydrate, 0.1 M Sodium malonate pH 7.0, 12.6 % (w/v) polyethylene glycol 4000 |
