8YPZ

Crystal strcture of human phosphoribosyl pyrophosphate synthetase 1 (PRPS1) in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9873
Spacegroup name	P 65
Unit cell lengths	186.876, 186.876, 160.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.700 - 3.000
Rwork	0.260
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h06
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	62270	6340
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	17.4
CC(1/2)	0.764	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M sodium acetate, 10% PEG 3350