8YOX

Crystal structure of hFSP1 with 6-OH-FAD (hFSP1-6-OH-FAD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	60.360, 60.360, 194.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.540 - 2.480
R-factor	0.1948
Rwork	0.191
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.922
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.590	2.540
High resolution limit [Å]	2.480	2.480
Number of reflections	15119	1005
<I/σ(I)>	19.3
Completeness [%]	98.4
Redundancy	14.9
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291.15	0.1 M Sodium chloride, 0.12 M Lithium sulfate, 0.1 M Sodium HEPES, 16% PEG 6000