8YNW
S102A mutant of poly(3-hydroxybutyrate) depolymerase PhaZ from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.989, 83.552, 85.729 |
| Unit cell angles | 73.48, 82.84, 83.62 |
Refinement procedure
| Resolution | 25.770 - 1.900 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ynv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.798 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.503 |
| Number of reflections | 91138 | 17026 |
| <I/σ(I)> | 13.97 | 3.6 |
| Completeness [%] | 97.4 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium acetate, 25 % PEG3350 |
