8YMZ

Structure of ZBTB43 in complex with CACA containing B-form DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.979, 67.535, 99.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 2.950
R-factor	0.2719
Rwork	0.269
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.101	0.066	1.088
Rmeas	0.106	0.070	1.141
Rpim	0.031	0.023	0.336
Total number of observations	109139
Number of reflections	8996	523	421
<I/σ(I)>	4.8
Completeness [%]	99.5	99.4	92.5
Redundancy	12.1	10.3	10.4
CC(1/2)	0.969	0.997	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M sodium chloride, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350