8YLS
Structure of SARS-CoV-2 Mpro in complex with its degrader
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.120, 80.820, 54.690 |
| Unit cell angles | 90.00, 116.50, 90.00 |
Refinement procedure
| Resolution | 59.190 - 1.930 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.190 | 1.990 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.026 | 0.225 |
| Number of reflections | 28213 | 4845 |
| <I/σ(I)> | 19.04 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.1 | 25 % w/v PEG 1500, 0.1 M SPG 9.0 |
