8YL9
Crystal structures of terpene synthases complexed with a substrate mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 146.990, 146.990, 146.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.320 - 2.750 |
| R-factor | 0.193 |
| Rwork | 0.187 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.222 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.320 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.135 | 0.515 |
| Number of reflections | 27686 | 3987 |
| <I/σ(I)> | 27.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 36.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200 mM Magnesium formate and 26% w/v PEG3350 |
