8YKU
PaThiL in complex with AMP-PNP and TMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953721724543 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 118.590, 118.590, 131.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.350 - 2.170 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (Version 1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.350 | 2.248 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rpim | 0.042 | |
| Number of reflections | 29549 | 2895 |
| <I/σ(I)> | 17.37 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 33.2 | |
| CC(1/2) | 0.998 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M calcium acetate hydrate, 0.1M sodium cacodylate pH 6.5, 40% (v/v) PEG 300 |
