8YK3
Blood group B alpha-1,3-galactosidase AgaBb from Bifidobacterium bifidum, construct T7-tag_24-673
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-06-29 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.340, 73.154, 177.968 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 47.880 - 3.500 |
| R-factor | 0.2072 |
| Rwork | 0.203 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.880 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 20905 | 4934 |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.760 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 15% (w/v) PEG 20000, 0.1M MES-NaOH pH 6.5 (Seed condition: 10% (w/v) PEG 6000, 0.1M citric acid pH 5.0, 4 degrees celsius) |
