8YK2
Blood group B alpha-1,3-galactosidase AgaBb from Bifidobacterium bifidum, construct T7-tag_24-700
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2012-06-24 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.557, 125.055, 196.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.800 - 1.960 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.803 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 126369 | 6186 |
<I/σ(I)> | 15.1 | |
Completeness [%] | 100.0 | |
Redundancy | 7.4 | |
CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 1.6M ammonium sulfate, 0.1M HEPES pH 7.5, 0.1M NaCl |