8YK1

Blood group B alpha-1,3-galactosidase AgaBb from Bifidobacterium bifidum, construct 23-844

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-02-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0520
Spacegroup name	C 1 2 1
Unit cell lengths	127.689, 68.771, 291.306
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	48.590 - 2.020
R-factor	0.1907
Rwork	0.189
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.590	2.060
High resolution limit [Å]	2.020	2.020
Number of reflections	161615	5145
<I/σ(I)>	8.9	2.7
Completeness [%]	98.2
Redundancy	7.1
CC(1/2)	1.000	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% (w/v) PEG 3350, 0.1M sodium iodide (seed condition: 10% (w/v) PEG 6000, 0.1M citric acid pH 5.0)