8YJN
Structure of E. coli glycyl radical enzyme YbiW with bound glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 114.926, 207.040, 210.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.840 - 2.420 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.067 | 0.495 |
| Rmeas | 0.072 | 0.531 |
| Rpim | 0.026 | 0.193 |
| Total number of observations | 358154 | 34768 |
| Number of reflections | 47693 | 4632 |
| <I/σ(I)> | 16.9 | 3.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.5 | 7.5 |
| CC(1/2) | 0.999 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Imidazole pH 8.0, 1 M sodium acetate |
