8YJM

Structure of human SPT16 MD-CTD and MCM2 HBD chaperoning a histone H3-H4 tetramer and a single chain H2B-H2A chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	191.337, 191.337, 80.094
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	165.700 - 4.150
R-factor	0.374
Rwork	0.263
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.504
Data scaling software	Aimless
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.700	4.640
High resolution limit [Å]	4.150	4.150
Number of reflections	12882	3605
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	8.6
CC(1/2)	0.998	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M sodium acetate, 0.1 M sodium citrate pH 5.5, 5%(w/v) PEG 4000