8YJ8
Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-22 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 292.931, 58.083, 50.760 |
Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
Resolution | 35.570 - 1.650 |
R-factor | 0.1895 |
Rwork | 0.188 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hh8 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.070 | 1.116 |
Rpim | 0.043 | 0.670 |
Number of reflections | 101614 | 3190 |
<I/σ(I)> | 8.4 | 1.1 |
Completeness [%] | 99.3 | 98.05 |
Redundancy | 3.72 | 3.69 |
CC(1/2) | 0.997 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M Ammonium sulfate, 20% w/v PEG 3,350 |