8YJ7

Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-01
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.591, 129.272, 245.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 2.800
R-factor	0.2243
Rwork	0.222
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hh8
RMSD bond length	0.008
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.530	2.920
High resolution limit [Å]	2.800	2.820
Rmerge	0.209	1.178
Rpim	0.082
Number of reflections	57771	2603
<I/σ(I)>	10
Completeness [%]	99.9
Redundancy	7.38
CC(1/2)	0.994	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.05 M Zinc acetate dihydrate, 20% w/v PEG 3350