8YJ6
Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-01 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.2820 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.935, 49.828, 147.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 1.370 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19370 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.821 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.17_3644+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.550 | 1.420 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.074 | 0.823 |
Rpim | 0.021 | 0.343 |
Number of reflections | 54591 | 592 |
<I/σ(I)> | 21 | 1.6 |
Completeness [%] | 87.4 | |
Redundancy | 12.3 | |
CC(1/2) | 0.999 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M MgCl2, 0.1 M Tris (pH 8.5), 25% w/v PEG3350 |