8YJ6

Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-01
Detector	RAYONIX MX225-HS
Wavelength(s)	1.2820
Spacegroup name	P 21 21 21
Unit cell lengths	39.935, 49.828, 147.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 1.370
R-factor	0.1711
Rwork	0.170
R-free	0.19370
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.17_3644+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.550	1.420
High resolution limit [Å]	1.370	1.370
Rmerge	0.074	0.823
Rpim	0.021	0.343
Number of reflections	54591	592
<I/σ(I)>	21	1.6
Completeness [%]	87.4
Redundancy	12.3
CC(1/2)	0.999	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M MgCl2, 0.1 M Tris (pH 8.5), 25% w/v PEG3350