8YJ5
Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-01 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 120.450, 120.450, 156.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.790 - 3.660 |
R-factor | 0.2651 |
Rwork | 0.263 |
R-free | 0.30330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hh8 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.037 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.790 | 3.790 |
High resolution limit [Å] | 3.660 | 3.660 |
Rmerge | 0.177 | 1.891 |
Rpim | 0.069 | 0.746 |
Number of reflections | 12349 | 3077 |
<I/σ(I)> | 7.9 | 1 |
Completeness [%] | 99.9 | |
Redundancy | 7.61 | |
CC(1/2) | 0.996 | 0.452 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 1.8 M Ammonium citrate tribasic |