8YIV

N17.1.2 recognition of NRAS neoantigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	71.144, 133.223, 222.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.692 - 2.101
R-factor	0.1925
Rwork	0.191
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	387710
<I/σ(I)>	13.7
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.992	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10mg/ml, 8% TacsimateTM pH7.0, 20% PEG4K