8YHW
The Crystal Structure of NF-kB-inducing Kinase (NIK) from Biortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.18079 |
Spacegroup name | P 41 |
Unit cell lengths | 119.652, 119.652, 115.586 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.560 - 2.900 |
Rwork | 0.194 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 3.030 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 36217 | 4398 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 100.0 | |
Redundancy | 9.4 | |
CC(1/2) | 0.992 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MMT pH9.0, 25% PEG1500 |