8YHW

The Crystal Structure of NF-kB-inducing Kinase (NIK) from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.18079
Spacegroup name	P 41
Unit cell lengths	119.652, 119.652, 115.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 2.900
Rwork	0.194
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.179
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	3.030
High resolution limit [Å]	2.900	2.900
Number of reflections	36217	4398
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.992	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MMT pH9.0, 25% PEG1500