8YHP
Structure of the PGK1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18075 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.591, 70.058, 95.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.300 - 1.950 |
| Rwork | 0.234 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.742 |
| Number of reflections | 30843 | 2162 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Na/K PO4, 20% PEG 3,350 |
