8YGY
Structure of the KLK1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.180820 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.708, 68.590, 80.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.401 |
| Rwork | 0.180 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.167 | 0.981 |
| Number of reflections | 9127 | 513 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 88.1 | |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M (NH4)2SO4, 0.1M Bis-Tris pH6.5, 25% PEG3,350. |
