8YFZ
CRYSTAL STRUCTURE OF THE EST1 H274E MUTANT AT PH 4.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-22 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.522, 71.672, 136.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.340 - 1.500 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.355 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.292 |
| Number of reflections | 46125 | 46125 |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 293 | 12% PEG 3000, 200MM NACL, 100MM PHOSPHATE-CITRATE, PH 4.2, VAPOR DIFFUSION, TEMPERATURE 293K |
