8YEY

TRIP4 ASCH domain in complex with ssDNA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 2 1
Unit cell lengths	38.077, 29.438, 126.462
Unit cell angles	90.00, 89.91, 90.00

Refinement procedure

Resolution	42.154 - 2.000
R-factor	0.2042
Rwork	0.201
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.929
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.119	0.089	0.430
Rmeas	0.133	0.102	0.491
Rpim	0.058	0.048	0.230
Total number of observations	98282
Number of reflections	19378	1040	869
<I/σ(I)>	6.1
Completeness [%]	99.0	97.9	92.9
Redundancy	5.1	4.9	4.1
CC(1/2)	1.000	0.987	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1 M Bicine (pH 8.5) and 20% (w/v) PEG 10,000