8Y6D
Norovirus GII.10 P domain and 2'-FL (tablet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.408, 78.926, 70.074 |
| Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
| Resolution | 43.550 - 1.410 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.550 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.060 | 1.261 |
| Rmeas | 0.067 | 1.435 |
| Rpim | 0.031 | 0.673 |
| Total number of observations | 608864 | 26159 |
| Number of reflections | 133866 | 6219 |
| <I/σ(I)> | 9 | 0.7 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.5 | 4.2 |
| CC(1/2) | 0.998 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.2 M sodium nitrate 0.1 M bis-tris propane (pH 7.5) 20% (w/v) PEG3350 |
