Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-02 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.925, 106.424, 83.002 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 41.250 - 1.860 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.076 | 0.684 |
| Rpim | 0.042 | 0.374 |
| Number of reflections | 88150 | 8773 |
| <I/σ(I)> | 12.49 | 2.5 |
| Completeness [%] | 100.0 | 99.84 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 0.998 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Potassium formate, Polyethylene glycol 3350 |
