8Y1U

Crystal structure of ASB7-Elongin B/C bound to the LZTS1-degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-12-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978610
Spacegroup name	P 1 21 1
Unit cell lengths	48.145, 43.724, 159.233
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	48.120 - 2.410
R-factor	0.217
Rwork	0.215
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.210	2.496
High resolution limit [Å]	2.410	2.410
Rmerge	0.093	0.846
Number of reflections	49898	4433
<I/σ(I)>	14.47
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M DL-Malic acid pH7.0, 10% w/v polyethylene glycol 3350