8Y06
Crystal structure of LbCas12a in complex with crRNA and 12nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 155.863, 155.863, 212.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.600 - 3.990 |
| R-factor | 0.2475 |
| Rwork | 0.246 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xuz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.686 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 3.990 | 3.990 |
| Rpim | 0.040 | 0.212 |
| Number of reflections | 25893 | 1236 |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 99.8 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M NaAc, pH 5.6, 12.5% PEG 2000MME |
