8XY0
Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-02 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.179, 41.783, 80.873 |
Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
Resolution | 44.270 - 1.900 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.068 | 0.348 |
Rmeas | 0.078 | 0.434 |
Number of reflections | 26490 | 3206 |
<I/σ(I)> | 13.05 | 2.28 |
Completeness [%] | 94.4 | 71.3 |
Redundancy | 3.87 | 2.46 |
CC(1/2) | 0.998 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.9 | 293 | 0.2 M ammonium iodide, 26% w/v PEG4,000 |