8XV7
Crystal structure of TTD-PHD domain of UHRF1 in complex with hStella peptide (residues 75-121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 |
| Unit cell lengths | 153.548, 153.548, 61.311 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.258 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.770 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.115 | 0.869 |
| Rmeas | 0.124 | 0.942 |
| Rpim | 0.045 | 0.358 |
| Total number of observations | 574184 | 31753 |
| Number of reflections | 76760 | 4582 |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.5 | 6.9 |
| CC(1/2) | 0.997 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 4% v/v Tacsimate pH 7.0, 12% PEG 3350 |
